soluble il 6rα  (R&D Systems)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Maresin 1 attenuates pro‐inflammatory activation induced by β‐amyloid and stimulates its uptake

doi: 10.1111/jcmm.16098

Figure Lengend Snippet: (A‐D) MaR1 reduced Aβ 42 ‐induced secretion of pro‐inflammatory cytokines in d‐THP‐1 cells. Differentiated THP‐1 (d‐THP‐1) cells were incubated with vehicle, 5 µM Aβ 42 , 5 µmol/L MaR1 or 5 µM Aβ 42 + 5 µM MaR1 for 24 h and the supernatants were analysed by ELISA. A total of 14 experiments were performed. MaR1 reduced the Aβ 42 ‐induced increase in interleukin (IL)‐1β (A), tumour necrosis factor (TNF)‐α (B) and IL‐6 (C). The levels of IL‐6 receptor (R) α (D) were not affected by Aβ 42 nor MaR1. Analysis of variance (ANOVA) was performed with the non‐parametric Kruskal–Wallis (K‐W) test, using the built‐in post hoc test for multiple comparisons to find significant differences between treatments. *** P < 0.005 vs. vehicle. # P < 0.05, ## P < 0.01, ### P < 0.005 vs. 5 μM Aβ 42 . Aβ = β‐amyloid; MaR1 = maresin 1

Article Snippet: Human tumour necrosis factor (TNF)‐α, IL‐1β, IL‐6, soluble IL‐6 receptor alpha (IL‐6Rα) and IL‐1 receptor type II enzyme‐linked immunosorbent assay (ELISA) kits and recombinant human monocyte chemoattractant protein (MCP)‐1 protein were purchased from R&D Systems (Abingdon, United Kingdom).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Maresin 1 attenuates pro‐inflammatory activation induced by β‐amyloid and stimulates its uptake

doi: 10.1111/jcmm.16098

Figure Lengend Snippet: (A‐C) MaR1 reduced Aβ 42 ‐induced pro‐inflammatory cytokine secretion in human MdM. Human monocyte‐derived microglia (MdM) were incubated with vehicle, 5 µM Aβ 42 , 5 µM MaR1 or 5 µM Aβ 42 + 5 µM MaR1 for 24 h. The levels of interleukin (IL)‐1β (A), tumour necrosis factor (TNF)‐α (B) and IL‐6 (C) were determined in the cell supernatants using a V‐PLEX human pro‐inflammatory panel. A total of 5 experiments were performed. Aβ 42 increased the levels of IL‐1β (A), TNF‐α (B) and IL‐6 (C), while co‐incubation with MaR1 reduced the elevation (A‐C). Analysis of variance (ANOVA) was performed with the Kruskal–Wallis (K‐W) test followed by pair‐wise comparisons of groups using Mann‐Withney with Bonferroni correction for multiple comparisons. * P < 0.05 vs. vehicle. # P < 0.05 vs. 5 μM Aβ 42 . Aβ = β‐amyloid; MaR1 = maresin 1

Article Snippet: Human tumour necrosis factor (TNF)‐α, IL‐1β, IL‐6, soluble IL‐6 receptor alpha (IL‐6Rα) and IL‐1 receptor type II enzyme‐linked immunosorbent assay (ELISA) kits and recombinant human monocyte chemoattractant protein (MCP)‐1 protein were purchased from R&D Systems (Abingdon, United Kingdom).

Techniques: Derivative Assay, Incubation